G418是一种氨基糖苷类抗生素 ,在分子遗传试验中，是稳定转染**常用的抗性筛选试剂。它通过抑制转座子Tn601，Tn5 的基因,干扰核糖体功能而阻断蛋白质合成，对原核和真核等细胞产生毒素 ,包括细菌、酵母、植物和哺乳动物细胞 ,也包括原生动物和蠕虫。当neo基因被整合进真核细胞DNA后 ,则能启动neo基因编码的序列转录为mRNA ,从而获得抗性产物氨基糖苷磷酸转移酶的高效表达 ,使细胞获得抗性而能在含有G418的选择性培养基中生长。G418的这一选择特性 ,已在基因转移、基因敲除、抗性筛选以及转基因动物等方面得以广泛应用。
technical data:
|
|
ITEM |
SPECIFICATION |
RESULT |
|||
|
Appearance |
White powder |
conforms |
|
|||
|
Solubility (Turbidity) 100mg/mL in H2O |
Clear to Very Slightly Hazy |
conforms |
|
|||
|
Biological Assay |
ED50 Resistant: ≥2500 ug/mL ED50 Sensitive: ≤400 ug/mL |
conforms |
|
|||
|
Absorbance: |
1mg/mL H2O:280nm < 0.015 100mg/Ml H2O: 570nm <0.10 |
conforms |
|
|||
|
pH |
4.6-6.0 |
5.6 |
|
|||
|
Heavy metals |
≤100 ppm |
90 ppm |
|
|||
|
Purity by HPLC
|
≥90% |
94.97% |
|
|||
|
** (by Karl Fischer) |
< 10 % |
3.0 % |
|
|||
|
Potency (on a dry basis) |
Not less than 720 ug/mg |
740 ug/mg |
|
|||
CONCLUSION: THE RESULTS CONFORM WITH ENTERPRISE STANDARD
G418 硫酸盐CAS号：108321-42-2
G418 硫酸盐分子式： C20H44N4O18S2
G418 硫酸盐英文名称： Geneticin
G418 硫酸盐英文同义词： ANTIBIOTIC G-418 SULFATE; G-418 SULPHATE; G 418; G418 SULFATE; G418 SOLUTION; G418 DISULFATE CELL CULTURE TESTED; G418 DISULFATE PLANT CELL CULTURE TESTED; (2R,3S,4R,5R,6S)-5-amino-6-[(1S,2R,4S,6R)-4,6-diamino-3-[(3R,4R,5R)-3,5-dihydroxy-5-methyl-4-methylamino-tetrahydropyran-2-yl]oxy-2-hydroxy-cyclohexoxy]-2-(1-hydroxyethyl)tetrahydropyran-3,4-diol; sulfuric acid
G418 硫酸盐分子量： 692.7094
G418 硫酸盐沸点： 1012.1°C at 760 mmHg
G418 硫酸盐密度： 0mmHg at 25°C
G418 硫酸盐闪点： 565.9°C
氨基糖苷类
申请入驻
