生化试剂

G-418硫酸盐,Geneticin,108321-42-2

G418是一种氨基糖苷类抗生素 ,在分子遗传试验中，是稳定转染最常用的抗性筛选试剂。它通过抑制转座子Tn601，Tn5 的基因,干扰核糖体功能而阻断蛋白质合成，对原核和真核等细胞产生毒素 ,包括细菌、酵母、植物和哺乳动物细胞 ,也包括原生动物和蠕虫。当neo基因被整合进真核细胞DNA后 ,则能启动neo基因编码的序列转录为mRNA ,从而获得抗性产物氨基糖苷磷酸转移酶的高效表达 ,使细胞获得抗性而能在含有G418的选择性培养基中生长。G418的这一选择特性 ,已在基因转移、基因敲除、抗性筛选以及转基因动物等方面得以广泛应用。   technical data:      ITEM                    SPECIFICATION RESULT Appearance White powder conforms   Solubility (Turbidity) 100mg/mL in H2O Clear to Very Slightly Hazy conforms   Biological Assay ED50 Resistant: ≥2500 ug/mL ED50  Sensitive: ≤400 ug/mL conforms   Absorbance: 1mg/mL H2O:280nm < 0.015 100mg/Ml H2O: 570nm <0.10 conforms   pH 4.6-6.0 5.6   Heavy metals ≤100 ppm 90 ppm   Purity by HPLC   ≥90% 94.97%   ** (by Karl Fischer)    < 10 % 3.0 %   Potency (on a dry basis) Not less than 720 ug/mg 740 ug/mg   CONCLUSION: THE RESULTS CONFORM WITH ENTERPRISE STANDARD

95%，97%

 For more about us , please visit our site: https://afinechem.en.alibaba.com or https://afinepharm.en.alibaba.com/   GENETICIN / G418 Sulfate  CAS 108321-42-2        Appearance: white to off-white powder Molecular Weight: 692.7 Density: g/cm3 Boiling Point: 1012.1°Cat760mmHg Flash Point: 565.9°C Storage Temperature: 2-8°C Solubility: soluble